Establishment and application of triple PCR method for CPV, CCoV and CRV

Canine parvovirus (CPV), canine coronavirus (CCoV) and canine rotavirus (CRV) are the three main causative viruses of diarrhea in dogs with similar clinical symptoms;thereby it is necessary to establish a high effective molecular detection method for differentiating the above pathogens. By optimizing the primer concentration and annealing temperature, a triple PCR method was established for simultaneous detection of CPV, CCoV and CRV, and then the specificity, sensitivity and repeatability of the method were tested. The results showed that the target fragments of CPV VP2 gene (253 bp), CCoV ORF-1b gene (379 bp) and CRV VP6 gene (852 bp) could be accurately amplified by the triple PCR method with high specificity, the detection limits of CPV, CCOV and CRV were 6.44x10-1 pg/L, 8.72x10-1 pg/L and 8.35x10-1 pg/L respectively with high sensitivity, and the method had good stability. Using this triple PCR method, 135 canine diarrhea fecal samples collected in Chengdu region from 2019 to 2020 were detected, and compared with those of single PCR method. The detection rates of CPV, CCoV and CRV were 16.30%, 20.74% and 4.44%, respectively, and the total infection rate was 51.11% (65/135) with 20.00% (13/65) co-infection rate. The detection results were consistent with three single PCR methods. In conclusion, CPV/CCoV/CRV triple PCR method successfully established in this paper can be applied as an effective molecular method to detection of related pathogens and to the epidemiological investigation.

Clinical and epidemiologic features of SARS-CoV-2 in dogs and cats compiled through national surveillance in the United States

OBJECTIVE: To characterize clinical and epidemiologic features of SARS-CoV-2 in companion animals detected through both passive and active surveillance in the US. ANIMALS: 204 companion animals (109 cats, 95 dogs) across 33 states with confirmed SARS-CoV-2 infections between March 2020 and December 2021. PROCEDURES: Public health officials, animal health officials, and academic researchers investigating zoonotic SARS-CoV-2 transmission events reported clinical, laboratory, and epidemiologic information through a standardized One Health surveillance process developed by the CDC and partners. RESULTS: Among dogs and cats identified through passive surveillance, 94% (n = 87) had reported exposure to a person with COVlD-19 before infection. Clinical signs of illness were present in 74% of pets identified through passive surveillance and 27% of pets identified through active surveillance. Duration of illness in pets averaged 15 days in cats and 12 days in dogs. The average time between human and pet onset of illness was 10 days. Viral nucleic acid was first detected at 3 days after exposure in both cats and dogs. Antibodies were detected starting 5 days after exposure, and titers were highest at 9 days in cats and 14 days in dogs. CLINICAL RELEVANCE: Results of the present study supported that cats and dogs primarily become infected with SARS-CoV-2 following expo- sure to a person with COVID-19, most often their owners. Case investigation and surveillance that include both people and animals are necessary to understand transmission dynamics and viral evolution of zoonotic diseases like SARS-CoV-2.

The animal keeping culture and the motivation of animal keepers in Hungary

Background: In the past few decades the animal keeping culture and habits have changed a lot internationally, some animals have become family members. The COVID-19 pandemic has created an unprecedented situation in the world, including Hungary. In many places, the quarantine period was associated with Isolation and, therefore, an increase in the number of companion animals. Objectives: Our aims were to (1) survey the animal keeping culture and habits, (2) examine the motivation of the owners and (3) explore the attitudes about adoption, neutering, breeding and the use of livestock animals in Hungary. Materials and Methods: Specialized surveys were conducted among 843 persons through social media and personal interviews from 20 June to 30 August 2021 by using a questionnaire including 16 questions. The chosen groups were compared with Fisher's Exact test and Chi-square test. Results and Discussion: 97.0% of the respondents regard their dog as a family member. Nearly 25% of the respondents do not wish to neuter their dogs, because they want to breed the dogs or they find it unnecessary. The primary reason for cat keepers not to neuter their pet is the financial issue. Animal keepers and women are significantly more likely to consider their animal as a family member (p < 0.001). Men, the elderly, people who live in the countryside and those who have only elementary education think that neutering is of less importance (p < 0.001). Non-animal keepers, elderly people and men think more negatively about adoption. Buying an animal from a breeder is more important to the younger age groups (p < 0.001) arid those who live in Budapest (p < 0.01). 42.3% of the respondents gave the lowest evaluation scores to the assessment of the livestock units. Women, people from younger age groups (p < 0.05) and those who have higher education (p < 0.001) think more negatively about the use of farm animals. Non-animal keepers, the elderly and people of lower education consider the livestock animals' emotional intelligence lower (p < 0.05). Men (p < 0.001), people over 65 years of age (p < 0.001) and of lower education (p < 0:01), and also non-animal keepers (p < 0.001) accept significantly easier the use of livestock animals.

Influence of the COVID-19 pandemic on dog ownership and rabies vaccination in Metropolitan Lima.

The aim of this study was to determine the possible influence of the COVID-19 pandemic on dog ownership and anti-rabies vaccination practices in Metropolitan Lima (Peru), taking as a source of information the National Survey of Budgetary Programmes (ENAPRES) carried out by the Instituto Nacional de Estadistica e Informatica (INEI) in the period 2017-2021. The proportion of respondents who owned at least one dog per household had an increasing trend, reaching the highest proportions in the pandemic years (2020: 47.5%;2021: 50.8%). The districts of Lima Sur and Lima Centro presented the highest (52.1-58%) and lowest (31.6-42.4%) proportion of respondents with dogs, respectively. According to the socioeconomic stratum (ESE), dog ownership had an inverse relationship (rs=-0.95), with a higher proportion in sector E (51.5-60.7%) and lower in sector A (32.5-43.1%). The proportion of vaccinated animals had a decreasing trend, reaching the lowest coverage during the pandemic years (2020: 84.8%;2021: 82.4%). The highest proportion of vaccinated dogs was found in Lima Centro (88.1-95.9%). According to ESE, the relationship was direct (rs=0.813). The highest proportion of vaccinated animals during the pre-pandemic and pandemic years occurred in ESE A (90.3-95%) and B (87.8-94.8%). In all the years of the study, the lowest proportions of vaccinated animals were in ESE E (75-80.2%). The study shows that dog ownership and rabies vaccination coverage were affected by the COVID-19 pandemic. The results can be used to assess the sanitary measures implemented to keep the capital free of urban rabies.Copyright © 2023 Universidad Nacional Mayor de San Marcos. All rights reserved.

"Sniffing" out SARS-CoV-2 in Arizona working dogs: an exploratory serosurvey.

Susceptibility to and infection with SARS-CoV-2 in companion animals has been well-documented throughout the COVID-19 pandemic. Surveillance for the virus in dogs has largely been focused on household pets; however, other canine populations may also be impacted. We partnered with a local veterinary hospital with a high working dog patient volume to conduct viral and neutralizing antibody testing in working dogs and identify potential risk factors in the dog's work and home environments. Surveillance of SARS-CoV-2 in law enforcement and security working dogs in Arizona found 24.81% (32/129) of dogs to be seropositive. Thirteen dogs presenting with clinical signs or with reported exposure to COVID-19 in the 30 days prior to sample collection were also tested by PCR; all samples were negative. 90.7% (n = 117) of dogs were reported to be asymptomatic or have no change in performance at the time of sampling. Two dogs (1.6%) had suspected anosmia as reported by their handlers; one of which was seropositive. Known exposure to the dog's COVID-19 positive handler or household member was identified as a significant risk factor. Demographics factors including sex, altered status, and type of work were not associated with canine seropositivity. Further work is warranted to understand the impact of SARS-CoV-2 and other infectious diseases in working dogs.

Scent dog identification of SARS-CoV-2 infections in different body fluids.

BACKGROUND: The main strategy to contain the current SARS-CoV-2 pandemic remains to implement a comprehensive testing, tracing and quarantining strategy until vaccination of the population is adequate. Scent dogs could support current testing strategies. METHODS: Ten dogs were trained for 8 days to detect SARS-CoV-2 infections in beta-propiolactone inactivated saliva samples. The subsequent cognitive transfer performance for the recognition of non-inactivated samples were tested on three different body fluids (saliva, urine, and sweat) in a randomised, double-blind controlled study. RESULTS: Dogs were tested on a total of 5242 randomised sample presentations. Dogs detected non-inactivated saliva samples with a diagnostic sensitivity of 84% (95% CI: 62.5-94.44%) and specificity of 95% (95% CI: 93.4-96%). In a subsequent experiment to compare the scent recognition between the three non-inactivated body fluids, diagnostic sensitivity and specificity were 95% (95% CI: 66.67-100%) and 98% (95% CI: 94.87-100%) for urine, 91% (95% CI: 71.43-100%) and 94% (95% CI: 90.91-97.78%) for sweat, 82% (95% CI: 64.29-95.24%), and 96% (95% CI: 94.95-98.9%) for saliva respectively. CONCLUSIONS: The scent cognitive transfer performance between inactivated and non-inactivated samples as well as between different sample materials indicates that global, specific SARS-CoV-2-associated volatile compounds are released across different body secretions, independently from the patient's symptoms. All tested body fluids appear to be similarly suited for reliable detection of SARS-CoV-2 infected individuals.

Infectious disease Roundup 2022

This paper describes the epidemiology, prevalence, transmission, prevention and control of some infectious diseases in companion animals, livestock, wild animals and humans in Ontario, Canada, in 2022, including SARS-CoV-2;Echinococcus multilocularis, Leishmania spp. and SARS-CoV-2;antimicrobial stewardship resources;2 cases of rabid dogs imported from Iran (July 2021 and January 2022);prevalence of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriacea, Dirofilaria immitis, Brucella canis, canine parainfluenza and adeno- and herpes viruses in dogs recently imported from Asia;Paragonimus kellicotti lung flukes and Streptococcus equi subsp. zooepidemicus in dogs;African swine fever in pet pigs, backyard pigs and wild pigs and blastomycosis in dogs and humans.

Epidemiology and Molecular Characterizations of Coronavirus from Companion Animals Living in Chengdu, Southwest China

The recent COVID-19 pandemic has once again caught the attention of people on the probable zoonotic transmission from animals to humans, but the role of companion animals in the coronavirus (CoV) epidemiology still remains unknown. The present study was aimed to investigate epidemiology and molecular characterizations of CoVs from companion animals in Chengdu city, Southwest China. 523 clinical samples from 393 animals were collected from one veterinary hospital between 2020 and 2021, and the presence of CoVs was detected by end-point PCR using pan-CoV assay targeting the RdRp gene. Partial and complete S genes were sequenced for further genotyping and genetic diversity analysis. A total of 162 (31.0%, 162/523) samples and 146 (37.2%, 146/393) animals were tested positive for CoVs. The positive rate in rectal swabs was higher than that in eye/nose/mouth swabs and ascitic fluid but was not statistically different between clinically healthy and diseased ones. Genotyping identified twenty-two feline enteric coronavirus (FCoV) I, four canine enteric coronavirus (CECoV) I, fourteen CECoV IIa, and one CECoV IIb, respectively. Eight complete S genes, including one canine respiratory coronavirus (CRCoV) strain, were successfully obtained. FCoV strains (F21071412 and F21061627) were more closely related to CECoV strains than CRCoV, and C21041821-2 showed potential recombination event. In addition, furin cleavage site between S1 and S2 was identified in two strains. The study supplemented epidemiological information and natural gene pool of CoVs from companion animals. Further understanding of other functional units of CoVs is needed, so as to contribute to the prevention and control of emerging infectious diseases.

Animals Other than Human Animals and Their Claim to Equal Consideration in Coronavirus and Criminological Study: Examining Harm to Domesticated Dogs during COVID-19 in the UK

While the financial and social support afforded to United Kingdon (UK) ‘animals other than human animals' (AOTHAs) welfare charities, such as the RSPCA and Dogs Trust, could suggest that they are valued intrinsically within Western society criminological texts largely omit them from recognition, with some important exceptions, see non-speciesist criminology. Most human animals likely do not want to directly or indirectly harm AOTHAs and even value relationships with "companion” AOTHAs. Regardless, AOTHAs have been victimized throughout history and continue to be. This article examines harm to AOTHAs in the context of the global COVID-19 pandemic to argue that abuse proliferates where harmful subjectivities are generated by society's acceptance of (1) the anthropocentric culture, and (2) when humanity values their individual advancement within the competitive consumer culture. Companion dogs were specifically focused upon within this article due to their so called close emotional and physical proximity to human animals, with proximity meaning that they were directly impacted by the lockdown measures implemented. The coronavirus pandemic was addressed by governments throughout the world by initiating an array of social restrictions. Because of these social restrictions, millions of individuals within England, and in countries such as the United States of America (USA), decided to adopt or purchase dogs for a variety of reasons, including to help them mitigate feelings of isolation and loneliness and to provide them with an excuse to participate in outdoor exercise. In order to determine the impact that the coronavirus pandemic has had upon the plight of domesticated companion dogs within England, semi-structured interviews, document analyses, and observation research were undertaken. The initial analysis of data presented here suggests that the coronavirus pandemic threatened the wellbeing of dogs within England, with their reproductive, physical, medical, and psychological wellbeing being put at risk.

How COVID has changed veterinary medicine

The COVID 19 pandemic has caused an increase in the number of owned cats and dogs and the resulting increased demand for veterinarians and veterinary services. This has also caused increased work stress and performance issues by veterinarians. This paper describes how veterinarians can minimize work stress by modifying their schedule of medical activities in order to still maintain an ideal work life balance.

Mad Dogs and Other New Yorkers: Rabies, Medicine, and Society in an American Metropolis, 1840-1920. Jessica Wang

I greatly admire how Wang covers shifting medical and popular knowledge around rabies, illustrating how different and conflicting knowledges structured responses to the threat of mad dogs at the individual, social, and governmental levels. Rabies was never as common or as deadly as this pandemic, but fear of the disease shaped lives and public health policy in New York City in countless ways between 1840 and 1920. Wang did not write for this moment, but the book has much to offer to students and scholars trying to make sense of a multitude of issues surrounding Covid-19 and public health. [Extracted from the article] Copyright of Journal of the History of Medicine & Allied Sciences is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)

Whole-Genome Characterization of SARS-CoV-2 Reveals Simultaneous Circulation of Three Variants and a Putative Recombination (20B/20H) in Pets, Brazzaville, Republic of the Congo.

Following the emergence of SARS-CoV-2, cases of pets infected with variants circulating among humans were reported. In order to evaluate the occurrence of SARS-CoV-2 circulation among pets in the Republic of the Congo, we conducted a ten-month study of dogs and cats living in COVID-19-positive households in Brazzaville and neighboring localities. Real-time PCR and the Luminex platform were used to detect SARS-CoV-2 RNA and antibodies to SARS-CoV-2 RBD and S proteins, respectively. Our results show for the first time the simultaneous circulation of several variants of SARS-CoV-2, including viruses from clades 20A and 20H and a putative recombinant variant between viruses from clades 20B and 20H. We found a high seroprevalence of 38.6%, with 14% of tested pets positive for SARS-CoV-2 RNA. Thirty-four percent of infected pets developed mild clinical signs, including respiratory and digestive signs, and shed the virus for about one day to two weeks. These results highlight the potential risk of SARS-CoV-2 interspecies transmission and the benefits of a "One Health" approach that includes SARS-CoV-2 diagnosis and surveillance of viral diversity in pets. This approach aims to prevent transmission to surrounding wildlife as well as spillback to humans.

Development of the pet owner connectedness scale (POCS)

Studies that have reported on the loneliness protective effect of pet ownership have recently been called into question owing to methodological drawbacks, including the use of inappropriate scales of measurement for loneliness in pet owner samples. It has also been recently demonstrated that pet interactions may only influence positive, not negative, human affect. In light of these recent advances, the current study aimed to create a new scale focusing on the inverse experience of loneliness: connectedness. An initial 24-item scale was developed using qualitative data collected from our previous study investigating the impact of pet ownership for Australians living alone during a government-enforced lockdown in response to COVID-19. Study 1 included 934 dog and cat owners who self-selected into the online study. Factor analyses revealed two distinct factors, and only items that loaded uniquely on one factor were retained. Study 2 included 526 dog and cat owners who self-selected into the online study, which confirmed the two-factor structure and established validity and reliability of the scale. The result was a 14-item Pet Owner Connectedness Scale (POCS) with two subscales: (i) owner-pet connection, and (ii) connectedness through pet. Hence, we present evidence for a new scale that can be used to measure the positive social states of connectedness that owners may gain from their pets. This may provide a good alternative to more traditional methods such as measuring the buffering effect pets have on negative social states such as loneliness. It may also offer a more robust method to measure the impact of pet interactions on their owners. (PsycInfo Database Record (c) 2022 APA, all rights reserved)

Dogs unleashed: The positive role dogs play during COVID-19

The unprecedented COVID-19 pandemic has forced the global community to cope with widespread fear and isolation. However, there are outpourings of kindness and community despite these challenging times. In some cases, mandatory "social distancing" has even strengthened our relationships. One such relationship is the bond between humans and dogs. In this commentary, I leverage visual autoethnography to detail and understand human-dog relationships in the time of COVID-19, highlighting their importance. I then critique the allocation of space for dogs and the rigid restrictions on dogs in public space. Finally, I use my experience with my dog as a lens to question the heavy policing of public space and reflect on broader issues of this enforcement. (PsycInfo Database Record (c) 2023 APA, all rights reserved)

Understanding the performance of truffle dogs

Trained dogs can detect many biochemical traces, from the most hostile, such as COVID-19, to the most expensive, such as truffles. However, it is still unclear how the interplay of genetic disposition, education, experience, personality, and environmental conditions influences the performance of dogs. Here, we combine published evidence with the knowledge of truffle hunters to assess direct and indirect factors that affect the hunting performance of truffle dogs. Since the transport of truffle aroma into the canine nasal cavity is most puzzling, we propose 5 experiments to further investigate the role of weather conditions, soil parameters, and bacterial composition on the dispersal of truffle volatiles perceived by trained dogs. We also suggest exploring whether the application of multiple dogs increases the success of truffle hunting. Moreover, we argue that interdisciplinary research on dog behavior and performance, including insights from veterinary science and comparative psychology as well as the experience of truffle hunters and farmers, is needed to improve the quantity and quality of truffle harvests. (PsycInfo Database Record (c) 2023 APA, all rights reserved)

Differentiating infectious from non-infectious respiratory disease in the cat and dog

This paper describes the clinical signs and use of differential laboratory diagnostic techniques (computed tomography, cytology, histopathology, antigen/antibody detection and polymerase chain reaction) for infectious (viral, bacterial, fungal and parasitic) and non-infectious (inflammatory/immune mediated, neoplastic, cardiac, malformation, foreign body, smoke inhalation, aspiration of caustic material, non-cardiogenic, pulmonary oedema, traumativ, pneumothorax, pulmonary contusions and idiopathic) causes of respiratory diseases in cats and dogs in Ontario, Canada.

Brachycephalic obstructive airway syndrome: an overview

One of the conformational issues by the explosion Of pet ownership throughout the COVID 19 pandemic is the Ming number dunes of brachycephalic obstructive envay syndrome (BOAS), which is a condition prevalent in some of the UK's most copular dog breeds The challenges the veterinary profession is not only to identify and treat affected individuals from within the population of dogs presenting to primary care clinicians, using surgical and non-surgical options but also to educate clients on how to recognise clinical signs of the disease as early as possible.

Seroepidemiological and clinicopathological investigation of Canine Coronavirus infection in dogs, in t..rkiye

Domestic and wild dogs of all ages and breeds are susceptible to Canine Coronavirus(CCoV) infections and be seen in T..rkiyeand amongst world. CCoV has recently been declared a zoonotic disease agent and the eighth pathogenic human coronavirus. This study was conducted on 143 naturally infected dogs with gastroenteritis which were not vaccinated against CCoV in T..rkiye in 2015-2020. The data of dogs were analyzed seroepidemiologically, clinicopathologically and statistically. CCOV antibodies in serum and CCOV antigens in stool were detected by ELISA and lateral immunochromatography. The rising CCoV IgG antibody titers were detected at all dogs and were as follows;<10 ng/L in 3 (2%), 10-20 ng/L in 18 (13%), 20-30 ng/L in 16 (11%), 30-40 ng/L in 14 (%10), 40-64 ng/L in 11 (8%) and >64 ng/L in 81 (81%) dogs. CCOV and Canine Parvovirus (CPV) antigen were detected together in the stool of the 41 (28.7%) dogs. As a result, it was concluded that the CCOV agent is in circulation among dogs living in T..rkiye. CCOV and CPV can cause co-infections and increased mortality. Although infection can be seen in dogs of all ages, it can be seen more frequently in dogs younger than 1 year of age, and especially in dogs younger than 6 months, and can cause enteritis, low hemoglobin, erythropenia, lymphopenia, leukopenia, thrombocytopenia, and hypoproteinemia.

SARS-CoV-2 in animals used for fur farming: GLEWS+ risk assessment (20 January 2021)

SARS-CoV-2 was first identified in humans in December 2019 and has since affected almost 68 million people causing over 1.5 million deaths worldwide. Animal-to-human and animal-to-animal transmission has been documented within farmed minks in several countries. SARS-CoV-2 has been identified in a farmed mink population in a number of countries. Some of the affected farms reported also workers SARS-CoV-2 infection and it is hypothesized that the mink farms were infected through human-mink transmission proving SARS-CoV-2 capability of reverse zoonosis. This Tripartite Risk Assessment, as a joint effort under the GLEWS+ initiative, completed with the Food and Agriculture Organization (FAO), the World Health Organization (WHO), and the World Organisation for Animal Health (OIE), evaluates the risk of introduction and spread of SARS-CoV-2 within fur farming systems as well as whether farmed fur animals could play a significant role in the spread of SARS-CoV-2 to humans via spillover. Additionally, using a One Health approach, the Tripartite evaluated the risk of the escaped minks leading to the establishment of a viral reservoir in susceptible wildlife populations. This work provides guidance to Members on this newly emerging threat.

The Promise of Disease Detection Dogs in Pandemic Response: Lessons Learned From COVID-19.

One of the lessons learned from the coronavirus disease 2019 (COVID-19) pandemic is the utility of an early, flexible, and rapidly deployable disease screening and detection response. The largely uncontrolled spread of the pandemic in the United States exposed a range of planning and implementation shortcomings, which, if they had been in place before the pandemic emerged, may have changed the trajectory. Disease screening by detection dogs show great promise as a noninvasive, efficient, and cost-effective screening method for COVID-19 infection. We explore evidence of their use in infectious and chronic diseases; the training, oversight, and resources required for implementation; and potential uses in various settings. Disease detection dogs may contribute to the current and future public health pandemics; however, further research is needed to extend our knowledge and measurement of their effectiveness and feasibility as a public health intervention tool, and efforts are needed to ensure public and political support.